RESUMEN
Madariaga virus (MADV), the new species designation for the South American isolates of eastern equine encephalitis virus (EEEV), is genetically divergent and substantially different in ecology and pathogenesis from North American EEEV strains. We isolated and characterized a MADV isolate obtained from a horse in Brazil. Our results support previous phylogenetic studies showing there are three genetically distinct MADV lineages. The MADV isolate from Paraíba State belongs to the South American lineage III and is closely related to Peruvian, Colombian and Venezuelan isolates.
Asunto(s)
Virus de la Encefalitis Equina del Este , Encefalomielitis Equina/veterinaria , Enfermedades de los Caballos/virología , Aedes/citología , Aedes/virología , Animales , Encéfalo/virología , Brasil , Células Cultivadas , Virus de la Encefalitis Equina del Este/clasificación , Virus de la Encefalitis Equina del Este/genética , Virus de la Encefalitis Equina del Este/aislamiento & purificación , Encefalomielitis Equina/virología , Caballos , Ratones , FilogeniaAsunto(s)
Factor VIII/administración & dosificación , Hemofilia A/parasitología , Malaria Vivax/complicaciones , Choque/etiología , Antimaláricos/uso terapéutico , Transfusión de Eritrocitos , Hemofilia A/tratamiento farmacológico , Humanos , Malaria Vivax/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Plasmodium vivax/aislamiento & purificación , Plasmodium vivax/metabolismo , Choque/terapia , Resultado del TratamientoRESUMEN
Thrombocytopenia may be occasionally observed in dengue fever (DF) but is a constant feature and one of the diagnostic criteria of dengue hemorrhagic fever (DHF). Its clinical presentation and relevance is still poorly described in the literature. Patients with fever and bleeding were referred to a tertiary care center in Manaus, in the Western Brazilian Amazon during the outbreak of dengue in 2001. They were hospitalized for clinical observation and supportive care. Platelet counts were performed on admission and throughout the hospitalization. Only patients with a serological confirmation or viral isolation of the dengue virus were included. One hundred and seventy eight patients were enrolled in the study (118 with DF and 60 with DHF). There was no association between the presence of active bleeding and the degree of thrombocytopenia on admission (P = 0.302). A positive tourniquet test, hematemesis and ecchymoses were more frequent in DHF patients (P < 0.05). Patients with DHF had lower platelet counts than patients with DF (P < 0.001). There was a positive correlation between platelet counts and plasma albumin levels (r = 0.217; P = 0.016) and a negative correlation with ALT values (r = -0.3; P = 0.001). Clinical manifestations of thrombocytopenia are not related only to the number of peripheral platelets in dengue infection, but its recovery is associated with clinical improvement. The level of platelets correlates with the vascular leakage of proteins and liver damage.
